Quantitation of DNA Sequences in Environmental PCR Products by a Multiplexed, Bead-Based Method
نویسندگان
چکیده
منابع مشابه
A bead-based method for multiplexed identification and quantitation of DNA sequences using flow cytometry.
A new multiplexed, bead-based method which utilizes nucleic acid hybridizations on the surface of microscopic polystyrene spheres to identify specific sequences in heterogeneous mixtures of DNA sequences is described. The method consists of three elements: beads (5.6-microm diameter) with oligomer capture probes attached to the surface, three fluorophores for multiplexed detection, and flow cyt...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2002
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.68.2.1010-1013.2002